Skip to main content
. 2017 Dec 7;9(1):622–640. doi: 10.18632/oncotarget.23079

Figure 1. Phenotypic and genotypic analysis of adherent and suspension MDA-MB-468 cells.

Figure 1

(A) Adherent and suspension cells were photographed. (B) Number of adherent and suspension cells were directly counted at the indicated day. (C) Cell cycle of adherent and suspension cells was analyzed using flow cytometry at the indicated day. (D) Total RNAs were isolated, and EMT and CSCs marker genes were amplified using RT-PCR. (E) Stably expressing luciferase MDA-MB-468 adherent and suspension cells were injected into the mammary fat pad of athymic nu/nu mice and the in vivo bioluminescent signal was quantified using IVIS Lumina XRMS. Representative in vivo images of adherent or suspension cells injected mice and a dot plot comparing the bioluminescent signal in each group (mean ± SEM, n = 6) are shown. *p < 0.05; two-tailed Mann Whitney t-test (F) Representative images of lung and liver tissue sections staining with vimentin in two groups of animals are shown (Scale bar = 100 µm). (G) The number of mice showing mammary tumor formation and metastases were indicated. AD, adherent cells; SUS, suspension cells.