Table 2. Correlation of PD-L1 and PD-L2 methylation and mRNA expression with immune cell infiltrates.
Analyte | B cells | T cells (CD4+) | T cells (CD8+) | Dendritic cells | ||||
---|---|---|---|---|---|---|---|---|
Spearman’s ρ | p-value | Spearman’s ρ | p-value | Spearman’s ρ | p-value | Spearman’s ρ | p-value | |
PD-L1 mRNA | 0.086 | 0.051 | 0.359 | <0.001 | 0.420 | <0.001 | 0.545 | <0.001 |
PD-L2 mRNA | 0.093 | 0.036 | 0.398 | <0.001 | 0.399 | <0.001 | 0.624 | <0.001 |
cg15837913 (PD-L1) | 0.024 | 0.59 | 0.007 | 0.88 | –0.197 | <0.001 | –0.086 | 0.052 |
cg02823866 (PD-L1) | 0.093 | 0.036 | 0.010 | 0.82 | –0.020 | 0.64 | –0.020 | 0.65 |
cg14305799 (PD-L1) | -0.071 | 0.11 | –0.111 | 0.012 | –0.122 | 0.006 | –0.112 | 0.011 |
cg13474877 (PD-L1) | -0.034 | 0.44 | –0.079 | 0.073 | –0.166 | <0.001 | –0.129 | 0.003 |
cg19724470 (PD-L1) | 0.099 | 0.025 | –0.010 | 0.83 | –0.136 | 0.002 | –0.079 | 0.073 |
cg14440664 (PD-L2) | 0.394 | <0.001 | 0.340 | <0.001 | 0.326 | <0.001 | 0.275 | <0.001 |
cg07211259 (PD-L2) | 0.091 | 0.040 | 0.053 | 0.23 | –0.065 | 0.14 | –0.008 | 0.86 |
Spearman’s correlation (ρ, p-value) between PD-L1 and PD-L2 promoter methylation together with PD-L1 and PD-L2 mRNA expression and immune cell infiltrates. Immune cell infiltrates were determined using RNA-Seq based quantification as shown by Li et al. [22].