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. 2017 Dec 7;9(1):668–679. doi: 10.18632/oncotarget.23089

Figure 2. Knockdown and overexpression of YAP were generated in CAL27 cells.

Figure 2

(A, B) RT- PCR was conducted to determine the mRNA levels of YAP and the downstream genes of Hippo pathway in CAL27 cells. (C, D) CAL27 cells with overexpression or knockdown of YAP were used to analyze the expression of YAP, p-YAP (Ser127), p-YAP (Ser397). GAPDH was used as a total control and Histone H3 was used as a nuclear control. *p < 0.05, **P < 0.01, ***p < 0.001.