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. 2017 Dec 12;9(1):755–765. doi: 10.18632/oncotarget.23146

Figure 2. Jazf1 enhances cell proliferation and colony formation ability in human prostate cancer cells.

Figure 2

(A) Jazf1-overexpressing human prostate cancer cell lines were established. The mRNA and protein levels of Jazf1 were measured upon Jazf1 overexpression. (B) A Jazf1 knock-down DU145 cell line was established. The mRNA and protein levels of Jazf1 were measured in both scrambled and Jazf1 knock-down DU145 cells. (C) The relative O.D. values of cell lines were measured in Jazf1-overexpressing cell lines using a CCK-8 assay. (D) The relative mRNA expression of Ki-67 was examined in Jazf1-overexpressing cell lines. (E) Relative O.D. values of Jazf1 knock-down DU145 cells were compared with scrambled DU145 cells. (F) The mRNA expression of Ki-67 was measured in Jazf1 knock-down DU145 cells. (G) Colony formation ability in Jazf1-overexpressing cell lines were compared with empty vector cell lines (50× magnification, scale bar = 100 µm). The numbers of colonies were counted in Jazf1-overexpressing cell lines. (H) Colony formation ability in Jazf1 knock-down DU145 cells was compared to that of scrambled DU145 cells (50× magnification, scale bar = 100 µm). The numbers of colonies were counted in Jazf1 knock-down DU145 cells. N1; pEGFP-N1 empty vector, shscr; shscrambled vector (Means ± SD, *p < 0.05, **p < 0.01 compared with control).