Figure 3. Jazf1 increases migration and invasion abilities.
(A) Migrated cells were stained with crystal violet in both Jazf1-overexpressing and knock-down cell lines (50× magnification, scale bar = 100 µm). (B) Cells that invaded through Matrigel were stained with crystal violet in both Jazf1-overexpressing and knock-down cell lines (50× magnification, scale bar = 100 µm). (C) The protein levels of EMT markers were measured in both Jazf1-overexpressing and knock-down cell lines using western blot. β-actin was measured as control. N1; pEGFP-N1 empty vector, shscr; shscrambled vector (Means ± SD, *p < 0.05, **p < 0.01 compared with control).