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. 2017 Dec 6;9(1):937–957. doi: 10.18632/oncotarget.22946

Figure 1. Hypoxia enhances mRNA expression of HIF-1 α and its target genes in ckit+CSCs.

Figure 1

Ckit+CSCs were incubated under normoxic or hypoxic conditions for 6, 24 and 48h. mRNA expression levels of (A) HIF-1 α, (B) VEGF, (C) GLUT1 and (D) EPO were determined by real time PCR. The bar graphs show fold increases in expression of the studied genes in ckit+CSCs with respect to control normoxic cells, set at 1. Data were normalized to GUSB, a housekeeping gene, and represent means ± SEM for three separate experiments, each repeated in triplicate. *, P < 0.05.