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. 2017 Dec 6;9(1):937–957. doi: 10.18632/oncotarget.22946

Figure 2. Hypoxia enhances mRNA expression of NFkB and markers of the NFkB-dependent inflammatory and reparative response in ckit+CSCs.

Figure 2

Ckit+CSCs were incubated under normoxic and hypoxic conditions for 6, 24 and 48h. mRNA expression levels for (A) RAGE, (B) P2X7R, (C) TLR2, (D) TLR4, (E) NFkB, (F) COX-2, (G) NOS2, (H) PTX3 and (I) CXCR4 were determined by real time PCR. The bar graphs show fold increases in expression of the studied genes in ckit+CSCs with respect to control normoxic cells, set at 1. Data were normalized to GUSB, a housekeeping gene, and represent means ± SEM for three separate experiments, each repeated in triplicate. *, P < 0.05.