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. 2018 Jan 24;9:47. doi: 10.3389/fimmu.2018.00047

Figure 1.

Figure 1

HD rapidly induces anti-HA neutralizing monoclonal antibody (mAb) in serum and in multiple mucosa. (A) Quantitative ELISA for neutralizing mAb in sera collected at indicated times after administration of the plasmid coding anti-HA IgG by electroporation (EP, n = 3) and by hydrodynamics (HD, n = 5). The naïve group represents no treatment (n = 5). Error bars represent the SEM. (B) Five days after HD or 20 days after EP, the serum (Naïve, n = 5, EP, n = 4, HD, n = 5), bronchoalveolar lavage without serum contamination (n = 5, n = 4, n = 4), urine (n = 3, n = 4, n = 3), and fecal (n = 3, n = 4, n = 5) specimens were obtained and subjected to quantitative ELISA. Each bar represents the median. The detection limit was over 46 ng/ml (serum) or 0.96 ng/ml (others). Each bar represents the median. ND, not detected. The indicated data about HD are representative of two independent experiments.