Figure 1.

G30 and G72 genes and pLG72 protein. (A) Nucleotide sequence of the G30 gene (genebank entry code AY138548.1); the gene is transcribed on the opposite DNA strand in comparison with G72. (B) Nucleotide sequence of the G72 gene; blue circles indicate nucleotides that are different in SNPs rs2391191 (AGG→AAG resulting in the R→K substitution at position 30) and rs9558562 (AAG→GAG resulting in the K→E substitution at position 62). The reported sequence codes for K30/K62 pLG72 variant (genebank entry code NM_172370.4 with A at position 89). (C) Primary and secondary structure of pLG72; residues preceding the limited proteolysis sites are shown in orange and residues modified by BS³ cross-linking reagent are shown in pink. Cross-linked K62 residue is shown in purple (Birolo et al., 2016). The secondary structure is shown according to the final homology model (blue) and as predicted by PSSpred server (purple).