Figure 1.

S. pyogenes culture supernatants induce cleavage of Dsg1 and Dsg3. Recombinant extracellular domains of Dsg1 and Dsg3 were separately incubated with culture supernatants from S. pyogenes clinical isolates for 3 h at 37°C. Sample proteins were separated by SDS-PAGE under a reducing condition, and subjected to western blot analysis using specific antibodies against the extracellular domain of Dsg1 and Dsg3.