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Elevated hydrostatic pressure impairs the activity of ADA, but not the protein levels of ADA and ADK. Total BV-2 cell extracts were assayed for ADA by Western blot (A) and enzymatic activity (B) as well as for ADK by Western blot (C). Representative images for ADA, ADK and GAPDH (loading control) are presented above the graphs (A,C). Results are expressed as percentage of control. *p < 0.05, different from control; Kruskal-Wallis test, followed by Dunn's multiple comparison test.
