Figure 5.

Elevated hydrostatic pressure changes the expression and density of A₁R but not A₃R. Total BV-2 cell extracts were assayed for A₁R (A) and A₃R (B) by Western blot. Representative images for A₁R, A₃R and GAPDH (loading control) are presented above the graphs. Results are expressed as percentage of control. Cells were immunolabelled with anti-A₁R (green) (C) or anti-A₃R (green) (D) antibodies. Phalloidin (red) staining was used to visualize cells. Nuclei were counterstained with DAPI (blue). Scale bar: 20 μm. ^*p < 0.05, different from control; Kruskal-Wallis test, followed by Dunn's multiple comparison test.