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. 2018 Jan 24;9:16. doi: 10.3389/fphar.2018.00016

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Copyright © 2018 Rodrigues-Neves, Aires, Vindeirinho, Boia, Madeira, Gonçalves, Cunha, Santos, Ambrósio and Santiago.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ATP and adenosine do not contribute to the increase of phagocytic efficiency in microglia induced by elevated hydrostatic pressure. Phagocytosis was assessed using fluorescent microbeads. (A) Representative images of BV-2 cells stained with phalloidin (red) with incorporated beads (green). Nuclei were counterstained with DAPI (blue). Arrowheads show some beads engulfed by microglia. (B) Phagocytic efficiency. Scale bar: 50 μm. ^**p < 0.01, different from control; Kruskal-Wallis test, followed by Dunn's multiple comparison test.