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Ach-induced relaxation in NE-precontracted aortic rings from 2 months-old (continuous line) and 18 months-old (discontinuous line) male (A) and female (B) mice. Values are means ± SEM; n = 6–8 vasoreactivity assays. #p < 0.01 old males vs. young males; *p < 0.05 old females vs. young females; &p < 0.01 old females vs. old males determined by Student's t-test.
