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Effect of ISO-treatment on vascular relaxation in aortic rings from 2 months-old male mice (A), 2 months-old female mice (B), 18 months-old male mice (C), and 18 months-old female mice (D). Values are means ± SEM; n = 6–8 vasoreactivity assays. ISO doses were 5, 40, 80, and 160 μg/g/d for 7 days. ISO, isoproterenol. *p < 0.05 vs. vehicle same group determined by two-way ANOVA and post hoc tests.
