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. 2017 Nov 14;3:17038. doi: 10.1038/celldisc.2017.38

Figure 4.

Figure 4

Bisulfite Sanger sequencing with four F1 hybrid ESCs confirmed the ASM at Hcn2/Polrmt locus and revealed the preferred hypomethylation of Cast allele. The ‘X’ indicates that a CpG site is not present in the allele(s). (a) The maternal (M) C57 allele and the paternal (P) Cast allele of a hybrid ESC line from a cross between C57 and Cast were hypermethylated and hypomethylated, respectively (top); The maternal Cast allele and the paternal C57 allele of a hybrid ESC line from a cross between Cast and C57 were hypomethylated and hypermethylated, respectively (bottom). (b) The maternal 129 allele and the paternal Cast allele of a hybrid ESC line from a cross between 129 and Cast were hypermethylated and hypomethylated, respectively (top); The maternal Cast allele and the paternal 129 allele of a hybrid ESC line from a cross between Cast and 129 were hypomethylated and hypermethylated, respectively (bottom). (c) DNA sequence alignment revealed a SNP-introduced binding motif for transcription factor MTF-1.