Figure 5.

Four scenarios for regions with bimodal methylation patterns and Park7 ASM. (a) Four scenarios to explain genomic regions bearing bimodal methylation patterns: bona fide imprinted ASM; genetics-dependent ASM that is independent of parent-of-origin; one allele with hypomethylation (or hypermethylation) in half of cells and the same allele with hypermethylation (or hypomethylation) in the remaining half of cells; and half of cells with biallelic hypermethylation and half of cells with biallelic hypomethylation. (b) In hybrid CastC57 ESC line, the maternal Cast allele (left) had half highly methylated and half lowly methylated PCR clones; similarly, the paternal C57 allele (right) had half highly methylated and half lowly methylated PCR clones. (c) In hybrid C57Cast ESC line, the maternal C57 allele (top) had half highly methylated and half lowly methylated PCR clones; similarly, the paternal Cast allele (bottom) had half highly methylated and half lowly methylated PCR clones.