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. 2018 Jan 24;9:11. doi: 10.3389/fimmu.2018.00011

Figure 1.

Figure 1

Effects ES L1 antigens on differentiation of dendritic cells (DCs). (A–E) Immature DCs were generated from monocytes in GM-CSF/interleukin-4 supplemented medium in the presence of ES L1 (10, 50, and 200 µg/ml) antigens, or their absence (control), during 5 days. (A) Representative histograms from the analysis of 7AAD+ (dead) cells after the culture are shown and, (B) the summarized results from three different donors are shown as mean% ± SD. (C) Representative plots for CD1a and CD14 expression on DCs after 5 days of culture with ES L1 are shown, and (D) the summarized results from three different donors are presented as mean% ± SD. (E) DCs differentiated in the presence or absence of ES L1, were stimulated with LPS/IFN-γ on Day 5, and the expression of CD83, CD86, CD40, and HLA-DR was analyzed by flow cytometry after 24 h. The results collected with three different DC donors are shown as mean ± SD (see also Figure S1 in Supplementary Material, showing a representative experiment). *p < 0.05, **p < 0.01, ***p < 0.005 compared to corresponding control DCs, or as indicated (one-way ANOVA with Tukey’s posttest).