Figure 3.

Expression studies of the accessory lipoproteins BamD_GC and BamE_GC in N. gonorrhoeae. A, wildtype N. gonorrhoeae FA1090 was cultured in liquid medium, and at the indicated time points, samples were withdrawn and processed for SDS-PAGE and immunoblotting analysis. B, quantities of BamD_GC and BamE_GC in wildtype FA1090 during in vitro conditions relevant to different infection sites (standard growth under aerobic conditions on solid medium (GCB), in the presence of normal human serum (+NHS), during iron deprivation (−Iron), and under anaerobiosis (−O₂)) were assessed by probing the whole-cell lysates with respective antibodies. C, 37 strains of N. gonorrhoeae, as indicated above the immunoblots, were grown concurrently on solid medium for 20 h in 5% CO₂ at 37 °C, and bacteria were collected, lysed, and processed for immunoblotting. In all experiments, samples containing the whole-cell lysates were matched by equivalent A₆₀₀ units, resolved in a 4–20% Tris-glycine gel, and transferred onto nitrocellulose. Immunoblot analysis was performed using polyclonal rabbit antisera against BamD_GC and BamE_GC. Migration of a molecular mass marker (kDa) is indicated on the left.