Figure 5.

Loss of BamE_GC has significant effect on the N. gonorrhoeae cell envelope. Wildtype N. gonorrhoeae FA1090 (wt) and ΔbamE strains were harvested during midlogarithmic phase and subjected to proteome extraction to separate cell envelopes (CE) and naturally released membrane vesicles (MV). A, C, and D, samples containing purified cell envelopes and membrane vesicles were normalized by total protein concentration, separated by SDS-PAGE and either visualized by Coomassie Brilliant Blue G-250 staining (A) or transferred onto nitrocellulose membrane and probed with the indicated antibodies, and the tested protein's abundance was quantified by densitometry (C and D). B, quantification of membrane vesicles (MVs) was achieved by calculating the protein content in the membrane vesicles to 1.0 liter original culture volume/OD unit (mg liter⁻¹ OD unit⁻¹). Experiments were performed in three biological replicates. Mean values and corresponding S.D. (error bars) are presented. Migration of a molecular mass marker (kDa) is indicated on the left. * denotes TamA (8).