Figure 6.

Comparative time-dependent HDXMS traces, collected at 10 °C, for select peptides whose rates of exchange are faster with OS. The color coding of the HDXMS traces represents SLO in the absence of OS (black) and in the presence of OS (red). Middle, a partial structure of the catalytic domain is shown with a focus on the peptides affected by the presence of OS. The color coding of the peptides refers to their unique HDXMS behaviors and represents residues 239–256 and 257–273 (salmon), 297–305 and 306–316 (wheat), 317–334 (pale yellow), and 751–761 (pale green). The number of the N-terminal amino acid of each peptide, affected by OS, is shown in the structure for reference. Insets, the HDXMS traces are reproduced but are plotted with the x axis in logarithmic scale.