Figure 1.

F508del-CFTR screening assay. A, screening procedure. CFBE41o⁻ cells co-expressing F508del-CFTR and a halide-sensitive YFP were reverse-transfected with siRNAs (final concentration, 30 nm). The cells were incubated at 37 °C prior to analysis. The assay was carried out 48 h after transfection, using a plate reader, and activity was estimated according to YFP fluorescence quenching by iodide in the presence of forskolin (20 μm) plus genistein (50 μm) (fsk + gen). Representative traces showing iodide influx in the presence or absence of F508del-CFTR function rescue are shown on the right. B, results of siRNA library screening. Each dot represents CFTR activity following silencing of a single gene. Genes are grouped in functional classes. C, graph reporting the ordered distribution of CFTR activity scores displayed in B.