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. 2017 Nov 20;293(4):1203–1217. doi: 10.1074/jbc.M117.816595

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Figure 5. — Effect of FAU knockdown on wild-type CFTR and TMEM16A function and expression. A, the scatter dot plots show wt-CFTR (left panel) and TMEM16A (right panel) activity in CFBE41o⁻ cells based on the YFP assay after transfection with indicated siRNAs (final concentration, 30 nm). The activity measured upon treatments was normalized for the activity detected under control condition (NT-siRNA). B, electrophoretic mobility of wt-CFTR (upper panel) and TMEM16A (lower panel) proteins in three different preparations of CFBE41o⁻ cells after transfection with anti-FAU siRNA (final concentration, 30 nm). C, detection by cell surface biotinylation of CFTR forms expressed at the plasma membrane. Immunoblot detection of CFTR and control proteins in the biotinylated fraction (left panel) and in total lysates (right panel) is shown. Absence of the cytosolic proteins calnexin (CNX) and 14-3-3 in the biotinylated fraction confirms surface protein-specific labeling in each experiment.