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. 2017 Dec 1;293(4):1243–1258. doi: 10.1074/jbc.M117.804252

Table 3.

Kinetic parameters for the hydrolysis of pNP-Fuc, 3-FL, and Lewis X by recombinant cFase I and mutants

	pNP-Fuc				3-FL				Lewis X
	k_cat	K_m	k_cat/K_m	Activity decrease	k_cat	K_m	k_cat/K_m	Activity decrease	k_cat	K_m	k_cat/K_m	Activity decrease
	min⁻¹	mm	min⁻¹ mm⁻¹	-fold	min⁻¹	mm	min⁻¹ mm⁻¹	-fold	min⁻¹	mm	min⁻¹ mm⁻¹	-fold
Wildtype	8.4 ± 0.2	0.6 ± 0.05	13.9 ± 0.4	1	247.1 ± 9.2	2.3 ± 0.5	106.6 ± 7.6	1	1293.2 ± 44.1	1.3 ± 0.3	967.2 ± 78.6	1
D242A	(24.0 ± 4.9) ×10⁻³	17.4 ± 4.2	(1.4 ± 0.2) ×10⁻³	1 ×10⁴	ND	ND	ND		4.9 ± 0.4	9.7 ± 2.8	0.5 ± 0.06	1928
E302A	(22.2 ± 0.9) ×10⁻³	1.2 ± 0.1	0.019 ± 0.001	736	ND	ND	ND		13.8 ± 1.2	12.8 ± 2.1	1.1 ± 0.1	900
E315A	ND^a	ND	ND		1.1 ± 0.02	5.1 ± 0.5	0.2 ± 0.008	473	4.7 ± 0.1	2.8 ± 0.4	1.7 ± 0.09	585
C73S	8.6 ± 0.1	0.6 ± 0.04	13.3 ± 0.2	1	232.0 ± 2.8	5.3 ± 0.4	43.4 ± 1.1	2	2068.2 ± 23.4	2.6 ± 0.1	783.8 ± 14.7	1
C317A	0.8 ± 0.02	0.5 ± 0.06	1.6 ± 0.06	9	80.4 ± 3.4	3.0 ± 0.9	27.0 ± 3.0	4	544.5 ± 10.6	3.3 ± 0.3	166.0 ± 5.1	6
C317S	3.7 ± 0.3	0.6 ± 0.2	6.0 ± 0.6	2	213.0 ± 3.1	6.9 ± 0.6	30.7 ± 0.9	3	1744.0 ± 17.6	4.1 ± 0.2	422.6 ± 6.3	2
C467S	7.6 ± 0.1	0.5 ± 0.04	14.9 ± 0.4	0.9	239.6 ± 1.5	2.0 ± 0.1	122.1 ± 2.2	0.9	1525.6 ± 47.6	1.5 ± 0.3	1044.5 ± 64.8	0.9
249Δ265	1.3 ± 0.05	1.3 ± 0.1	1.0 ± 0.04	14	81.0 ± 2.2	4.1 ± 0.5	19.7 ± 0.9	5	1429.6 ± 94.8	13.2 ± 3.1	108.6 ± 9.2	9

^a ND means not detectable.