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. 2017 Dec 13;293(4):1271–1285. doi: 10.1074/jbc.M117.812693

Figure 4.

Figure 4.

Lost constitutive activity of N-truncated Orai1 mutants is restored by Orai3-loop2. a, time course of whole-cell inward currents at −74 mV activated by passive store depletion of HEK 293 cells expressing Orai1 P245L compared with Orai1 ΔN1–78 P245L and Orai1 ΔN1–78 Orai3-loop2 P245L. b, bar graphs comparing constitutive currents at t = 0 s after whole-cell break-in of Orai1 P245L, Orai1 ΔN1–78 P245L, Orai1 ΔN1–78 Orai3-loop2 P245L, Orai1 L74E/E76E/P245L, Orai1 L74E/E76E Orai3-loop2 P245L, Orai1 K85E/P245L, and Orai1 K85E/P245L Orai3-loop2. c, bar graphs comparing constitutive currents at t = 0 s after whole-cell break-in of Orai1 V181A, Orai1 ΔN1–78 V181A, Orai1 ΔN1–78 Orai3-loop2 V181A, Orai1 L185A, Orai1 ΔN1–78 L185A, and Orai1 ΔN1–78 Orai3-loop2 L185A. d, bar graphs comparing constitutive currents at t = 0 s after whole-cell break-in of Orai3 F160A, Orai3 ΔN1–53 F160A, and Orai3 ΔN1–57 F160A. e, bar graphs comparing constitutive currents at t = 0 s after whole-cell break-in of Orai3 P254L, Orai3 ΔN1–53 P254L, and Orai3 ΔN1–57 P254L. *, p < 0.05. Error bars, S.E.