Figure 3.

GALNT6 knockout induces crypt-like morphology in the LS174T colon cancer cell line. a, actin-lined lumens were detected by phalloidin staining (red) that was visualized by confocal microscopy. XY micrographs from three z-sections are presented. Scale bar, 20 μm. b, XZ micrographs of WT and ΔT6 colonies generated by confocal z-stacks. c, expression of GalNAc-T6 after re-introduction of GALNT6 (ΔT6+T6) in GALNT6 knockout cells (ΔT6). Green, GalNAc-T6; blue, DAPI. Scale bar, 10 μm. d, colon carcinoma and healthy tissue section stained with DAPI (blue). e, more than 300 colonies of each cell type were investigated, and the percentage of crypt-forming colonies was determined in a blinded manner. *, t test, p value = 0.008853.