Figure 5.

GALNT6 knockout increases cell–cell adhesion. a, mRNA expression of markers of epithelial-to-mesenchymal transition in LS174T WT, ΔT6, and ΔT6+T6 cells. b, mRNA expression of proteins involved in adhesion. c, fluorescence staining of E-cadherin and CD44 proteins in colonies of LS174T WT, ΔT6, and ΔT6+T6 cells (green, DAPI in blue). Scale bar, 20 μm. d and e, cells grown to confluency were dissociated from the plastic surface as complete cell layers by dispase treatment. After pipetting the cells up and down three times, cell fragments were counted as a measure of cell–cell adhesion. Fragments with at least 20 cells were counted. e, micrographs of the cell layers/fragments. Scale bars, 500 μm. *, t test, p value = 0.0436.