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. 2017 Nov 30;293(4):1373–1385. doi: 10.1074/jbc.M117.799536

Table 2.

Fitted values ± S.E. of parameters in empirical equations (Equations 11 and 12) to the results obtained at different [Rb+] and [ADP]

[ADP] [Rb+] aNaO12 aNa3 aNa4 aNa5 KNa12 KNa3 KNa4 KNa5 oNa12 oNa3 oNa4 aNa3/(KNa1 KNa2 KNa3) KNa1 KNa2 KNa3 aNa3/(oNa3/2) aNa4/(oNa4/2)
mm mm mol Pi/mol enzyme s mol Pi/mol enzyme s mol Pi/mol enzyme s mol Pi/mol enzyme s mm mm mm mm mol Rb+/mol enzyme mol Rb+/mol enzyme mol Rb+/mol enzyme (mol Pi/(mol enzyme s))/mm3 mm3 s1 s1
0 0.035 0.19 ± 0.03 3.02 ± 0.55 0.32 ± 0.07 6.6 × 103 ± 8.4 × 104 0.2 ± 0.1 0.9 ± 0.3 1.2 ± 0.3 1.1 × 106 ± 1.4 × 107 0.044 ± 0.001 0.60 ± 0.11 0.004 ± 0.001 132 ± 64 0.02 ± 0.01 10.1 ± 0.2 146 ± 41
0 0.120 0.28 ± 0.03 5.16 ± 0.45 1.76 ± 0.13 1.2 × 104 ± 7.8 × 104 0.3 ± 0.1 1.1 ± 0.2 4.6 ± 0.7 2.5 × 106 ± 1.7 × 107 0.066 ± 0.001 1.03 ± 0.09 0.027 ± 0.004 67 ± 20 0.08 ± 0.02 10.1 ± 0.2 130 ± 20
0 0.245 0.32 ± 0.03 7.17 ± 0.37 3.91 ± 0.26 5.7 × 104 ± 8.3 × 104 0.6 ± 0.1 0.8 ± 0.1 13.7 ± 1.9 1.4 × 107 ± 1.6 × 107 0.091 ± 0.002 1.42 ± 0.07 0.158 ± 0.014 28 ± 5 0.25 ± 0.04 10.1 ± 0.2 50 ± 6
0 0.500 0.25 ± 0.03 7.11 ± 0.42 9.32 ± 0.59 0.5 ± 8.5 1.0 ± 0.4 0.6 ± 0.1 14.2 ± 5.2 8.8 × 102 ± 7.98 × 102 0.165 ± 0.004 1.41 ± 0.08 0.648 ± 0.107 11 ± 3 0.64 ± 0.13 10.1 ± 0.2 29 ± 6
0 1 0.30 ± 0.03 6.20 ± 0.20 12.67 ± 0.17 1.3 ± 0.5 0.4 ± 0.1 15.1 ± 1.5 0.328 ± 0.005 1.23 ± 0.03 0.803 ± 0.017 10 ± 2 0.63 ± 0.11 10.1 ± 0.2 31.5 ± 0.9
0 3 0.16 ± 0.02 8.04 ± 0.28 15.85 ± 0.21 1.3 ± 0.3 0.6 ± 0.1 20.1 ± 2.3 0.883 ± 0.010 1.60 ± 0.04 1.420 ± 0.027 7 ± 1 1.08 ± 0.15 10.1 ± 0.2 22.3 ± 0.6
0 5 0.11 ± 0.03 8.29 ± 0.33 15.57 ± 0.20 1.5 ± 0.6 0.8 ± 0.1 17.2 ± 2.5 1.213 ± 0.010 1.65 ± 0.06 1.409 ± 0.27 5 ± 1 1.79 ± 0.44 10.1 ± 0.2 22.1 ± 0.6
2 0.010 0.07 ± 0.02 0.80 ± 0.42 0.03 ± 0.03 1.4 × 103 ± 4.8 × 104 0.3 ± 0.4 1.0 ± 1.0 0.8 ± 0.5 3.1 × 105 ± 1.0 × 107 0.033 ± 0.002 0.16 ± 0.14 11 ± 12 0.07 ± 0.06 5.8 ± 0.1
2 0.020 0.08 ± 0.03 1.87 ± 1.23 0.4 ± 0.4 1.4 ± 1.5 1.2 ± 1.1 0.040 ± 0.002 0.37 ± 0.42 0.026 ± 0.017 10 ± 7 0.19 ± 0.10 5.8 ± 0.1
2 0.035 0.04 ± 0.02 2.78 ± 0.78 0.02 ± 0.05 5.4 × 104 ± 8.9 × 104 0.3 ± 0.2 2.1 ± 0.9 1.7 ± 0.6 9.0 × 105 ± 1.5 × 107 0.046 ± 0.003 0.55 ± 0.27 0.007 ± 0.002 13 ± 8 0.22 ± 0.10 5.8 ± 0.1 7 ± 14
2 0.060 0.11 ± 0.04 3.22 ± 0.87 0.09 ± 0.21 0.5 ± 0.4 1.5 ± 0.7 4.2 ± 2.3 0.053 ± 0.004 0.64 ± 0.30 0.012 ± 0.055 8 ± 4 0.42 ± 0.15 5.8 ± 0.1 14 ± 39
2 0.120 0.12 ± 0.02 3.17 ± 0.30 1.00 ± 0.18 0.8 ± 0.3 1.2 ± 0.3 9.6 ± 1.7 0.068 ± 0.003 0.63 ± 0.10 0.036 ± 0.009 5 ± 1 0.69 ± 0.16 5.8 ± 0.1 55 ± 18
2 0.245 0.13 ± 0.02 2.67 ± 0.12 2.97 ± 0.15 1.3 ± 0.5 0.7 ± 0.1 30.8 ± 5.6 0.095 ± 0.004 0.53 ± 0.04 0.080 ± 0.028 2.4 ± 0.5 1.12 ± 0.22 5.8 ± 0.1 74 ± 27
2 1 0.12 ± 0.03 1.91 ± 0.12 6.25 ± 0.19 3.0 ± 3.3 0.3 ± 0.2 7.6 ± 1.3 0.322 ± 0.007 0.38 ± 0.04 0.549 ± 0.013 0.8 ± 0.3 2.48 ± 0.99 5.8 ± 0.1 22.8 ± 0.9
2 3 0.06 ± 0.02 2.28 ± 0.10 7.30 ± 0.21 4.5 ± 5.1 0.3 ± 0.2 10.1 ± 1.9 0.888 ± 0.014 0.45 ± 0.03 0.728 ± 0.017 0.4 ± 0.1 5.51 ± 2.00 5.8 ± 0.1 20.1 ± 0.8
2 5 0.03 ± 0.03 2.66 ± 0.09 8.04 ± 0.34 5.1 ± 5.7 0.4 ± 0.3 20.1 ± 4.6 1.189 ± 0.017 0.53 ± 0.02 0.785 ± 0.021 0.3 ± 0.1 9.22 ± 4.06 5.8 ± 0.1 20.5 ± 1.2