Table 2.
Fitted values ± S.E. of parameters in empirical equations (Equations 11 and 12) to the results obtained at different [Rb+] and [ADP]
| [ADP] | [Rb+] | aNaO12 | aNa3 | aNa4 | aNa5 | KNa12 | KNa3 | KNa4 | KNa5 | oNa12 | oNa3 | oNa4 | aNa3/(KNa1 KNa2 KNa3) | KNa1 KNa2 KNa3 | aNa3/(oNa3/2) | aNa4/(oNa4/2) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| mm | mm | mol Pi/mol enzyme s | mol Pi/mol enzyme s | mol Pi/mol enzyme s | mol Pi/mol enzyme s | mm | mm | mm | mm | mol Rb+/mol enzyme | mol Rb+/mol enzyme | mol Rb+/mol enzyme | (mol Pi/(mol enzyme s))/mm3 | mm−3 | s−1 | s−1 |
| 0 | 0.035 | 0.19 ± 0.03 | 3.02 ± 0.55 | 0.32 ± 0.07 | 6.6 × 103 ± 8.4 × 104 | 0.2 ± 0.1 | 0.9 ± 0.3 | 1.2 ± 0.3 | 1.1 × 106 ± 1.4 × 107 | 0.044 ± 0.001 | 0.60 ± 0.11 | 0.004 ± 0.001 | 132 ± 64 | 0.02 ± 0.01 | 10.1 ± 0.2 | 146 ± 41 |
| 0 | 0.120 | 0.28 ± 0.03 | 5.16 ± 0.45 | 1.76 ± 0.13 | 1.2 × 104 ± 7.8 × 104 | 0.3 ± 0.1 | 1.1 ± 0.2 | 4.6 ± 0.7 | 2.5 × 106 ± 1.7 × 107 | 0.066 ± 0.001 | 1.03 ± 0.09 | 0.027 ± 0.004 | 67 ± 20 | 0.08 ± 0.02 | 10.1 ± 0.2 | 130 ± 20 |
| 0 | 0.245 | 0.32 ± 0.03 | 7.17 ± 0.37 | 3.91 ± 0.26 | 5.7 × 104 ± 8.3 × 104 | 0.6 ± 0.1 | 0.8 ± 0.1 | 13.7 ± 1.9 | 1.4 × 107 ± 1.6 × 107 | 0.091 ± 0.002 | 1.42 ± 0.07 | 0.158 ± 0.014 | 28 ± 5 | 0.25 ± 0.04 | 10.1 ± 0.2 | 50 ± 6 |
| 0 | 0.500 | 0.25 ± 0.03 | 7.11 ± 0.42 | 9.32 ± 0.59 | 0.5 ± 8.5 | 1.0 ± 0.4 | 0.6 ± 0.1 | 14.2 ± 5.2 | 8.8 × 102 ± 7.98 × 102 | 0.165 ± 0.004 | 1.41 ± 0.08 | 0.648 ± 0.107 | 11 ± 3 | 0.64 ± 0.13 | 10.1 ± 0.2 | 29 ± 6 |
| 0 | 1 | 0.30 ± 0.03 | 6.20 ± 0.20 | 12.67 ± 0.17 | 1.3 ± 0.5 | 0.4 ± 0.1 | 15.1 ± 1.5 | 0.328 ± 0.005 | 1.23 ± 0.03 | 0.803 ± 0.017 | 10 ± 2 | 0.63 ± 0.11 | 10.1 ± 0.2 | 31.5 ± 0.9 | ||
| 0 | 3 | 0.16 ± 0.02 | 8.04 ± 0.28 | 15.85 ± 0.21 | 1.3 ± 0.3 | 0.6 ± 0.1 | 20.1 ± 2.3 | 0.883 ± 0.010 | 1.60 ± 0.04 | 1.420 ± 0.027 | 7 ± 1 | 1.08 ± 0.15 | 10.1 ± 0.2 | 22.3 ± 0.6 | ||
| 0 | 5 | 0.11 ± 0.03 | 8.29 ± 0.33 | 15.57 ± 0.20 | 1.5 ± 0.6 | 0.8 ± 0.1 | 17.2 ± 2.5 | 1.213 ± 0.010 | 1.65 ± 0.06 | 1.409 ± 0.27 | 5 ± 1 | 1.79 ± 0.44 | 10.1 ± 0.2 | 22.1 ± 0.6 | ||
| 2 | 0.010 | 0.07 ± 0.02 | 0.80 ± 0.42 | 0.03 ± 0.03 | 1.4 × 103 ± 4.8 × 104 | 0.3 ± 0.4 | 1.0 ± 1.0 | 0.8 ± 0.5 | 3.1 × 105 ± 1.0 × 107 | 0.033 ± 0.002 | 0.16 ± 0.14 | 11 ± 12 | 0.07 ± 0.06 | 5.8 ± 0.1 | ||
| 2 | 0.020 | 0.08 ± 0.03 | 1.87 ± 1.23 | 0.4 ± 0.4 | 1.4 ± 1.5 | 1.2 ± 1.1 | 0.040 ± 0.002 | 0.37 ± 0.42 | 0.026 ± 0.017 | 10 ± 7 | 0.19 ± 0.10 | 5.8 ± 0.1 | ||||
| 2 | 0.035 | 0.04 ± 0.02 | 2.78 ± 0.78 | 0.02 ± 0.05 | 5.4 × 104 ± 8.9 × 104 | 0.3 ± 0.2 | 2.1 ± 0.9 | 1.7 ± 0.6 | 9.0 × 105 ± 1.5 × 107 | 0.046 ± 0.003 | 0.55 ± 0.27 | 0.007 ± 0.002 | 13 ± 8 | 0.22 ± 0.10 | 5.8 ± 0.1 | 7 ± 14 |
| 2 | 0.060 | 0.11 ± 0.04 | 3.22 ± 0.87 | 0.09 ± 0.21 | 0.5 ± 0.4 | 1.5 ± 0.7 | 4.2 ± 2.3 | 0.053 ± 0.004 | 0.64 ± 0.30 | 0.012 ± 0.055 | 8 ± 4 | 0.42 ± 0.15 | 5.8 ± 0.1 | 14 ± 39 | ||
| 2 | 0.120 | 0.12 ± 0.02 | 3.17 ± 0.30 | 1.00 ± 0.18 | 0.8 ± 0.3 | 1.2 ± 0.3 | 9.6 ± 1.7 | 0.068 ± 0.003 | 0.63 ± 0.10 | 0.036 ± 0.009 | 5 ± 1 | 0.69 ± 0.16 | 5.8 ± 0.1 | 55 ± 18 | ||
| 2 | 0.245 | 0.13 ± 0.02 | 2.67 ± 0.12 | 2.97 ± 0.15 | 1.3 ± 0.5 | 0.7 ± 0.1 | 30.8 ± 5.6 | 0.095 ± 0.004 | 0.53 ± 0.04 | 0.080 ± 0.028 | 2.4 ± 0.5 | 1.12 ± 0.22 | 5.8 ± 0.1 | 74 ± 27 | ||
| 2 | 1 | 0.12 ± 0.03 | 1.91 ± 0.12 | 6.25 ± 0.19 | 3.0 ± 3.3 | 0.3 ± 0.2 | 7.6 ± 1.3 | 0.322 ± 0.007 | 0.38 ± 0.04 | 0.549 ± 0.013 | 0.8 ± 0.3 | 2.48 ± 0.99 | 5.8 ± 0.1 | 22.8 ± 0.9 | ||
| 2 | 3 | 0.06 ± 0.02 | 2.28 ± 0.10 | 7.30 ± 0.21 | 4.5 ± 5.1 | 0.3 ± 0.2 | 10.1 ± 1.9 | 0.888 ± 0.014 | 0.45 ± 0.03 | 0.728 ± 0.017 | 0.4 ± 0.1 | 5.51 ± 2.00 | 5.8 ± 0.1 | 20.1 ± 0.8 | ||
| 2 | 5 | 0.03 ± 0.03 | 2.66 ± 0.09 | 8.04 ± 0.34 | 5.1 ± 5.7 | 0.4 ± 0.3 | 20.1 ± 4.6 | 1.189 ± 0.017 | 0.53 ± 0.02 | 0.785 ± 0.021 | 0.3 ± 0.1 | 9.22 ± 4.06 | 5.8 ± 0.1 | 20.5 ± 1.2 |