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. 2017 Dec 7;293(4):1413–1424. doi: 10.1074/jbc.M117.814046

Figure 1.

Figure 1.

PROCR activates ERK, PI3K–Akt–mTOR, and RhoA–ROCK signaling pathways in breast cancer cells. a, illustration and representing image of phosphokinase antibody array using lysates of MDA-MB-231 cells with scramble control and PROCR-shRNA (Sh2). Seven proteins with most evident down-regulation following PROCR knockdown are indicated. b, Western blot showing down-regulation of ERK, PI3K–Akt–mTOR, and RhoA–ROCK pathway activities in MDA-MB-231 cells with PROCR-shRNA (Sh1 and Sh2) knockdown. Tubulin was used as loading controls. c, Western blot showing differential activities of ERK, PI3K–Akt–mTOR, and RhoA–ROCK pathway in PROCR–high TNBC cell lines (MDA-MB-231, Hs 578T) and PROCR–low TNBC cell lines (MDA-MB-468, BT549). Tubulin was used as loading control. Western blots in the same panel are from the same batch of cells using the same loadings, thus only one loading control is shown at the end of the panel. For a better illustration, they are shown as three separated columns representing ERK, Akt, and RhoA pathway, respectively. Each Western blot analyses was repeated for three times or more.