Figure 1.
The m.4435A→G mutation created the m1G37 modification of tRNAMet. A, cloverleaf structure of human mitochondrial tRNAMet. Arrows indicate the location of the m.4435A→G mutation. Solid lines represent the DIG-labeled oligonucleotide probe specific for tRNAMet. Broken lines represented the potential stops of primer extension caused by the m1G37 modification. B, primer extension demonstrated the creation of m1G37 in the tRNAMet carrying the m.4435A→G mutation. The primer extension termination products caused by m1G37 modification are showed.