Figure 11.

Measurement of ROS. Ratio of geometric mean intensity between levels of the ROS generation in the vital cells with or without H₂O₂ stimulation. The rates of production in ROS from three mutant cell lines and three control cell lines were analyzed by BD-LSR II flow cytometer system using MitoSox (5 μm) either without (A) or with (C) H₂O₂ stimulation. The relative ratio of intensity (stimulated or unstimulated with H₂O₂) was calculated. B and D, the average of three independent determinations for each cell lines is shown. Graph details and symbols are explained in the legend to Fig. 4.