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. 2017 Dec 8;293(4):1425–1438. doi: 10.1074/jbc.RA117.000317

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Figure 3. — The analysis of the stability of tRNA^Met. A, Northern blot analysis of tRNA under native conditions. Two μg of total mitochondrial RNA from various cell lines were electrophoresed through native polyacrylamide gels, electroblotted, and hybridized with DIG-labeled oligonucleotide probes specific for the tRNA^Met, tRNA^Thr, tRNA^Leu(CUN), and tRNA^Ser(AGY), respectively. B, thermal stability of wildtype (A37) and mutant (G37) tRNA^Met. ΔT_m indicates the difference of the T_m value between wildtype (A37) and mutant (G37) tRNA^Met. The calculations were based on three independent experiments.