Figure 6.

Heterologous competition between PIP‐47Aa and AfIP‐1A/AfIP‐1B to test for cross‐resistance to Cry34/Cry35. (a) A representative gel that depicts the specific binding of Alexa‐PIP‐47Aa (2 nM) and lack of competition by a saturating concentration of a mixture of AfIP‐1A (0.5 μm) and AfIP‐1B (2 μM). (b) A bar graph depicting the averaged densitometry values with standard error bars determined from gel images as shown in panel a. No significant reduction in Alexa‐PIP‐47Aa binding was observed during heterologous competition. Note that AfIP‐1B exists as an N‐ and C‐terminal fragment during binding, so densitometry values for both fragments were analysed to calculate the averaged values. (c) A representative gel depicting the specific binding and reciprocal competition when Alexa‐AfIP‐1B (10 nM) along with AfIP‐1A (500 nM) was incubated in the presence of a saturating concentration (8 μM) of PIP‐47Aa. (d) A bar graph depicting the averaged densitometry values with standard error bars determined from gel images as shown in panel c. No significant reduction in Alexa‐AfIP‐1B binding was observed during heterologous competition.