Figure 3.

Effect of dsRNA treatment of nematode cultured on carrot discs. The effect of dosing nematodes weekly with dsRNA molecules on (a,b) mean population density and (c,d) target transcript level was assessed after 8‐week culture on carrot discs for (a,c) Radopholus similis and (b,d) Pratylenchus coffeae. Dosing was with dsRNA molecules in M9 buffer targeting sequences of act‐4 (act‐4 and act‐4_t) or pas‐4. Quantitative RT‐PCR values are means ± SEM for four technical replicates of three biological replicates with significant loss of expression relative to nontargeting gfp dsRNA treatment indicated. Analysis is based on univariate ANOVA of log values (n = 10) with Tukey post hoc comparison to gfp, ***P < 0.001; **P < 0.01.