Table 1.
Potential target genes identified from database sequences
| Gene | Wormbase ID | C. elegans expression | C. elegans RNAi phenotype | Rs | Mi | Pc | Hm |
|---|---|---|---|---|---|---|---|
| ACT‐4 |
WBGene 00000066 |
Body wall and vulval muscles | Embryonic lethal | RSC00784 | MIC00350 | ✓ | ✓ |
| CCO‐1 |
WBGene 00000371 |
Mitochondrial | Lethal | RSC00550 | – | ✓ | – |
| HSP‐60 |
WBGene 00002025 |
Mitochondrial | Embryonic lethal | RSC02271 | MIC03015 | – | – |
| PAS‐3 |
WBGene 00003924 |
All major muscles | Embryonic lethal | RSC00234 | MIC05526 | ✓ | – |
| PAS‐4 |
WBGene 00003925 |
Pharynx and body wall muscle | Lethal | ✓ | MIC06652 | ✓ | ✓ |
| RPS‐23 |
WBGene 00004492 |
Muscles and nervous system | Embryonic lethal | RSC00184 | MIC03023 | – | – |
| UBQ‐1 |
WBGene 00006727 |
Muscles, hypodermis and nervous system | Lethal | RSC02466 | MIC06345 | – | ✓ |
| UBQ‐2 |
WBGene 00006728 |
Muscles and nervous system | Embryonic lethal | RSC00037 | MIC06345 | ✓ | – |
| UNC‐87 |
WBGene 00006819 |
All major muscles | Lethal | RSC04225 | MIC03833 | – | – |
RNAi phenotypes are the most severe given on Wormbase for C. elegans. The Nembase 4 gene reference is given for R. similis and M. incognita sequences obtained following a BLAST search with the C. elegans sequence. Sequences amplified from cDNA either had >80% sequence identity and 21‐nt regions with (✓) or without (–) 100% identity to other banana nematode sequences. The plant‐parasitic nematodes are as follows: R. similis (Rs), M. incognita (Mi), Pratylenchus coffeae (Pc) and Helicotylenchus multicinctus (Hm).