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. 2017 Sep 7;16(2):628–637. doi: 10.1111/pbi.12802

Figure 1.

Figure 1

Representation of the relevant constructs used in this work. pRIC3.0‐LALF 32‐51‐E7 and pRIC3.0‐cTP‐LALF 32‐51‐E7 are the initial constructs used to express LALF 32‐51‐E7 in N. benthamiana leaves. pEGFP contains the target EGFP sequence and a stop codon (black stars). pUC57‐LALF 32‐51‐E7 contains the plant codon‐optimized LALF‐E7 sequence, appropriate restriction sites for subcloning (shown by the light‐blue arrows), a rigid linker (PAPAP) and no His‐tag or stop codons. The final constructs pRIC3.0‐LALF 32‐51‐E7‐EGFP (LG) and pRIC‐cTP‐LALF 32‐51‐E7‐EGFP (cTP‐LG) were generated in a stepwise manner. Also shown are the cauliflower mosaic virus (CAMV) 35 S constitutive promoter and the chloroplast transit peptide (cTP). Not drawn to scale.