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. 2017 Sep 7;16(2):628–637. doi: 10.1111/pbi.12802

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© 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Colocalization profiles of LG and chloroplasts. Colocalization profiles were generated using the colocalization feature of the Zeiss Zen software. (a) Positive control, pRIC3.0‐EGFP. (b) Negative control, pRIC3.0 empty vector. (c) Cytoplasmic LG. (d) cTP‐LG. Green and red curves correspond to EGFP fluorescence and the chlorophyll autofluorescence, respectively. White bars represent the area used to generate the colocalization profiles. Magnification = 40×. Shown here are representatives of several images taken over two independent sets of experiments.