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Frequencies of CRISPR/Cas9‐induced mutations for tb1a, tb1b and PGM genes
| Gene | Callus line numbera | Number of sequenced independent transgenic events | Number of plants sequenced | Number of mutated plants | Mutation frequency (%) |
|---|---|---|---|---|---|
| tb1 | 7 | 32 | 46 | 44 (tb1a) | 95.6 |
| 5 (tb1b)b | 11 | ||||
| PGM | 1 | 1 | 3 | 0 | 13.7 |
| 3 | 1 | 19 | 5 | ||
| 5 | 8 | 25 | 2 | ||
| 13 | 4 | 4 | 0 |
Each individual callus line was derived from a single caryopsis.
tb1b mutant plants also carry the tb1a mutations.
