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. Author manuscript; available in PMC: 2018 Jan 29.
Published in final edited form as: Oncogene. 2016 Apr 4;35(42):5527–5538. doi: 10.1038/onc.2016.93

Fig 3. PRR14 promotes lung cancer cells growth.

Fig 3

siRNA was transfected into lung cancer cell line: H1299 and A549 respectively. Compared with control sequence, PRR14 specific siRNA decreased its mRNA level to less than 50% (A, D). Growth curves following transfection were monitored by Alamar Blue Assay (B, E). Colony formation was performed at the same time (C, F). Colony numbers were calculated and statistically analyzed by two-tailed student's t-test. The numbers were presented as mean±S.D (n=3). The overexpression of PRR14 in H1299 cells was confirmed by immunostaining (G). Then growth curve (H) and colony formation assay (I) were performed. Colony numbers were calculated and statistically analyzed by two-tailed student's t-test. The numbers were presented as mean±S.D (n=3).