Skip to main content
. Author manuscript; available in PMC: 2018 Jan 29.
Published in final edited form as: Oncogene. 2016 Apr 4;35(42):5527–5538. doi: 10.1038/onc.2016.93

Fig 6. PRR14 interacts with GRB2 to activate PI3K/Akt/mTOR signaling pathway.

Fig 6

Schematic diagram of the domain structures of GRB2 (A) and PRR14 (B) as well as the protein fragments used in this study. A direct interaction between PRR14 and full-length GRB2 or its fragments was shown by GST pull-down (C). Similarly, the direct interaction between GRB2 and PRR14 fragments was shown in (F). Co-immunoprecipitation of exogenous PRR14 and GRB2 as well as their fragments in HEK293 cells was shown in (D, E, G). (H) siRNA sequences targeting different sites of GRB2 were cotransfected with either PRR14 coding plasmid or its empty vector into H1299 cells and 48 hours later, cells were harvested for WB. (I) GFP-tagged PRR14 fragments 1–4 were transiently transfected into H1299 cells and cells were harvested for WB 24h later. (J) H1299 cell lines overexpressing GFP-tagged PRR14 fragments 1–4 were established and their proliferation rate and tumorigenesis were shown by growth curve (J) and colony formation assay (K).