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. 2018 Jan 29;9:5. doi: 10.1186/s40104-017-0228-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Genotyping results from single blastocysts injected with CRISPR/Cas9 system. All PCR products were digested with NheI. PCR product size from wild-type genomic DNA was 1.1 kb. If the embryo carried modified allele through HDR, we expected to see two fragments (950 bp and 160 bp) after digestion with NheI. Genomic DNA from an embryo carrying homozygous HDR mutation was served as a positive control (P). * indicates embryos carrying knock-in events. L is a molecular ladder