Table 3.
Experiment no. | Substrate | Laccase treated | Fermentation mode | Initial OD | SSC (g/l) | Total fermentation time (h) | Nitrogen source (g/l) |
---|---|---|---|---|---|---|---|
1 | Lignin 1 | No | Batch | 1.0 | 10 at 0 h | 96 | 1.4 |
2 | Lignin 1 | No | Batch | 10 | 40 at 0 h | 96 | 1.4 |
3 | Lignin 1 | No | Fed-batch | 10 | 20 at 0 h + 20 at 72 h | 168 | 1.4 |
4 | Lignin 4 | No | Batch | 1.0 | 10 at 0 h | 96 | 1.4 |
5 | Lignin 4 | No | Fed-batch | 5.0 | 20 at 0 h + 20 at 72 h | 168 | 1.4 |
6 | Lignin 4 | Yes | Fed-batch | 5.0 | 20 at 0 h + 20 at 72 h | 168 | 1.4 |
Lignins 1 and 4 represent the lignin substrate fractionated by pretreatment Cases 1 and 4, as described in Table 1, respectively
SSC soluble substrate concentration