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. 2018 Jan 24;11:5. doi: 10.3389/fnmol.2018.00005

FIGURE 6.

FIGURE 6

Kainic acid-induced ER stress promotes GSK-3 and CDK5 activities. (A) Relative activity of GSK-3 in KA-treated mouse hippocampus. (B) Relative activity of CDK5 in KA-treated mouse hippocampus. (C) Relative activity of CDK5 in KA, KA+Ros, and/or KA+SB -treated primary hippocampal neurons. The primary hippocampal neurons were pre-treated with or without SB (20 μM) or Ros (100 μM) for 1 h and then stimulated with KA (50 μM) for 8 h. (D) Relative activity of GSK-3 in KA, KA+Ros, and/or KA+SB -treated primary hippocampal neurons. (E) Relative activity of GSK-3 in KA and/or Sal-treated primary hippocampal neurons. The primary hippocampal neurons were pre-treated with or without Salubrinal (5 or 10 μM) for 1 h and then stimulated with KA (50 μM) for 8 h. (F) Relative activity of CDK5 in KA and/or Sal-treated primary hippocampal neurons. (G,I) Protein levels of GRP78, ATF-6, p-PERK, and IRE-1 in KA and/or SB-treated primary hippocampal neurons. (H,J) Protein levels of GRP78, ATF-6, p-PERK, and IRE-1 in KA and/or Ros-treated primary hippocampal neurons. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. controls; #P < 0.05; ##P < 0.01 vs. the KA group; the significant difference from the respective values were determined by one-way analysis of variance test. N = 3 for western blotting; n = 5 for assay of activity).