Figure 5.

ar^{uab105/uab105} ovaries have fewer mature oocytes than do wild-type ovaries. H&E staining of adult female (A) wild-type and (B) ar^{uab105/uab105} ovary sections. (A′, B′) High-magnification images of boxed areas in (A) and (B). Wild-type ovaries consist of a majority of mature late-stage III followed by stage II oocytes, with stage I oocytes in less abundance. ar^{uab105/uab105} ovaries contain primarily early-staged I and II and almost no stage III oocytes. Scale bar: (A, B) 200 μm, (A′, B′) 50 μm. (C) Quantification of oocyte number according to stage in wild-type and mutant ovaries. Each dot represents average number of oocytes per histological section per fish (n = 3 fish per genotype, 9 sections per fish). Significance is indicated with asterisks (****P < 0.0001; ns = not significant) from one-way ANOVA.