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. Author manuscript; available in PMC: 2018 Jan 29.
Published in final edited form as: Virology. 2014 Jul 30;464-465:156–165. doi: 10.1016/j.virol.2014.07.004

Fig. 5.

Fig. 5

The cell-mediated immune response upon vaccination with 1918 virus. (A) Groups of 3–5 mice are vaccinated i.m. with DK/96-wt and DK/96-Hu (10 or 2.5 μg) or SC/18-wt and SC/18-Av (2.5 or 0.25 μg) vaccines. Fourteen days later, spleen tissues are harvested and the frequency of virus-specific IgG+ASCs are measured by ELISPOT assay. The number of virus-specific IgG+ASCs is normalized against the number of total IgG+secreting ASCs and presented as % Ag-specific IgG+B cells. (B) Mice are vaccinated with DK/96-wt and DK/96-Hu (10 or 2.5 μg) or SC/18-wt and SC/18-Av (2.5 or 0.25 μg). Fourteen days post-immunization, spleen are harvested and the frequency of IFN-γ-producing CD4+ and IFN-γ-producing CD8+ T cells from immunized mice is measured by intracellular cytokine staining following 5 days in vitro culture after infection for 1 h with DK/96 or SC/18 virus. Statistical significance is determined by two-way ANOVA. ** indicates statistical significance (p<0.01) between Dk/96-Hu and Dk/96-wt vaccine, *** p<0.001.