Fig. 6.

The ability of nonstimulatory peptides to enhance antigen-recognition is independent of agonist strength. RMA-S cells were loaded with titrated amounts of antigen (OVA; a), a weaker agonist (Q4; b) or a very weak agonist (T4; c) in the absence or presence of various endogenous K^b-binding, OT-I nonstimulatory peptides (Santori et al. 2002). The upregulation of CD69 on prepositive selection thymocytes from OT-I transgenic Tap^−/− mice was assessed and expressed in relation to the expression of the OVA–K^b epitope of mAb 25-D1.16 mAb (Porgador et al. 1997). Used with permission from Yachi et al. (2007)