Table 1.

LC and MS conditions for the purification and the detection of catecholamines and monoamines and their respective heavy tagged counterparts.

HPLC gradient
Time (min)	0		2.5		4.5		6.5		7.5	8	10
% B buffer	1		1		30		99		99	1	1
MS ionization, selection, fragmentation, and identification parameters
Compound		Polarity		Precursor (m/z)		Product (m/z)		Ion product type				Collision energy (V)
Adenosine		Positive		268.25		136.07		Qualification and Quantification				26
C5-Adenosine		Positive		273.25		136.18		Qualification and Quantification				23
AccQ-Tag-D4-dopamine		Positive		328.25		171.16		Qualification and Quantification				35
AccQ-Tag-Dopamine		Positive		324.25		171.16		Qualification and Quantification				38
AccQ-Tag-C6-noradrenaline		Positive		346.25		171.16		Qualification and Quantification				35
AccQ-Tag-noradrenaline		Positive		340.25		171.16		Qualification and Quantification				35
AccQ-Tag-D6-adrenaline		Positive		360.25		171.16		Qualification and Quantification				36
AccQ-Tag-adrenaline		Positive		354.25		171.16		Qualification and Quantification				34
AccQ-Tag-D4-serotonin		Positive		351.25		171.16		Qualification and Quantification				40
AccQ-Tag-serotonin		Positive		347.25		171.16		Qualification and Quantification				38

Note: Buffer A corresponded to ACN 1%/H₂O 98.9%/formic acid 0.1% (v/v/v), whereas buffer B was ACN 99.9%/formic acid 0.1% (v/v).