Figure 4.

Effects of different concentrations of orientin on cell apoptosis in primary rat cortical neurons under OGD exposure for 6 h followed by reoxygenation for 24 h. (a) The levels of caspase-3, cleaved caspase-3, Bcl-2, Bax, and β-actin proteins were measured by western blotting and the representative blots were presented. (b) The caspase-3 activity was measured via the commercial kit. (c) The cell apoptosis rate was detected by flow cytometry analysis. *P < 0.05 versus Blank control, ^#P < 0.05 versus Control.