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. Author manuscript; available in PMC: 2018 Jan 29.
Published in final edited form as: Int Rev Cell Mol Biol. 2013;300:1–50. doi: 10.1016/B978-0-12-405210-9.00001-1

Figure 3.

Figure 3

Genetic lineage-tracing experiments. (A) Schematic representation of the double transgenic DNA construct used for lineage-tracing experiments. Tamoxifen administration translocates CreER to the nucleus, where Cre-mediated recombination takes place. (B) Example of lineage-tracing experiments using Tamoxifen-inducible Cre driven by spermatogonia-specific Nanos2 enhancer/promoter (Sada et al., 2009). Two days after Tamoxifen injection, spermatogonia located on the basement membrane are labeled. Three months after labeling, a sufficiently long period for a repeated completion of spermatogenesis, seminiferous tubule of testis contained all stages of spermatogenic germ cells.