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. Author manuscript; available in PMC: 2018 Jan 29.
Published in final edited form as: Int Rev Cell Mol Biol. 2013;300:1–50. doi: 10.1016/B978-0-12-405210-9.00001-1

Figure 7.

Figure 7

Intestinal stem cells. (A) The anatomy of the small intestinal epithelium. (B) Confetti reporter construct. Cre triggers both inversion and recombination in a random manner, which results in the four patterns of gene expression (nuclear GFP, cytoplasmic YFP and RFP, membrane-associated CFP). Cre-mediated inversion occurs at a sequence flanked by loxP sites in opposite orientation. In 50% of cells, inversion should lead to an antisense orientation and switch gene expression. (C) Multicolor lineage tracing shows a progressive monoclonality of the crypt. (D) Monoclonal conversion arises from turnover of an equipotent stem cell population. Paneth cells make a specialized microenvironment for intestinal stem cells.